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对68份不同进化阶段、不同种皮色稻种资源的储藏特性、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、还原型谷胱甘肽(GSH)、花色苷含量、1,2-二苯基苦基苯肼清除率(SFRC)进行测定,研究稻种储藏过程中体内自由基清除率、抗氧化相关酶类和非酶抗氧化物质的变化。结果表明,稻种资源的储藏特性、自由基清除率(SFRC)、抗氧化相关酶类和非酶抗氧化物质存在显著的基因型差异。老化指数与SFRC、花色苷、GSH、POD呈负相关,SFRC与SOD、CAT呈正相关,与种皮中花色苷含量呈极显著正相关(r=0.641**)。随着储藏时间的延长,其体内抗氧化酶(SOD、POD、CAT)活性逐渐降低,GSH、SFRC也呈下降的趋势,而花色苷含量变化不显著。该研究结果为利用稻种资源的有益基因,开展耐储藏专用型水稻品种选育奠定了基础。
The characteristics of storage characteristics, superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), reduced glutathione GSH), anthocyanin content and 1,2-diphenylpicylphenyl-hydrazine scavenging rate (SFRC) were measured to study the effects of free radical scavenging rate, antioxidative enzymes and non-enzymatic antioxidants Variety. The results showed that there were significant genotypic differences in the storage characteristics, free radical scavenging rate (SFRC), antioxidase related enzymes and non-enzymatic antioxidants of rice resources. The aging index was negatively correlated with SFRC, anthocyanin, GSH and POD. SFRC was positively correlated with SOD and CAT, and had a significant positive correlation (r = 0.641 **) with anthocyanin content in seed coat. With the prolongation of storage time, the activity of antioxidant enzymes (SOD, POD, CAT) gradually decreased, GSH and SFRC also decreased, while anthocyanin content changed insignificantly. The results of this study laid a foundation for breeding useful rice varieties with beneficial genes of rice resources.