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以黄瓜(Cucumis sativusL.)果皮有光泽自交系‘1101’(P1)为母本,以3个无光泽自交系‘1116’(P2)、‘9930’(P3)、‘1107’(P4)为父本,分别构建6世代遗传群体,对黄瓜果皮光泽性状进行遗传规律分析和基因定位研究。结果表明,黄瓜果皮有光泽性状由显性单基因G控制,有光泽对无光泽为显性。利用‘1101’ב1116’的F2群体,结合分离群体分组分析法(bulked segregate analysis,BSA)筛选得到了30对与黄瓜果皮有光泽基因G相关的SSR标记,将该基因定位到黄瓜第5染色体上,侧翼标记为CS28和SSR15818,遗传距离分别为2.0cM和6.4cM。两侧翼标记之间的物理距离为454kb,在该区域中共预测了177个候选基因。
The results showed that the three inbred lines “1116” (P2), “9930” (P3), “1107” (P4) ) Were used as paternal parents to construct 6-generation genetic groups respectively. The genetic traits and gene mapping of cucumber peel luster were studied. The results showed that the glossy traits of cucumber peel were controlled by the dominant single gene G, and the dullness of the cucumber was dominant. Thirty SSR markers were screened from bulked segregate analysis (BSA) of ’1101’ × ’1116’ in F2 populations and mapped to the g Chromosomes, flanked by CS28 and SSR15818, had genetic distances of 2.0 cM and 6.4 cM, respectively. The physical distance between the flanking markers was 454 kb, and a total of 177 candidate genes were predicted in this region.