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目的 :观察豚鼠咽鼓管鼓室粘膜受γ射线照射后损伤的显微形态学改变 ,对其放射损伤的病理学基础作初步研究。方法 :选择正常成年豚鼠 16只 ,耳颞部接受 6 0Gyγ射线照射。照射结束后 2、4、8及 12周分别处死动物各四只 ,用显微镜观察照射侧的中耳腔、咽鼓管、鼓室粘膜 ;对照组动物 4只不作照射 ,取鼓室粘膜和咽鼓管作正常对照。结果 :实验组全部照射鼓室腔均出现大量脓细胞 ,随后局部出现肉芽组织及纤维组织 ;鼓室粘膜上皮完全脱落 ;粘膜下组织水肿 ,血管壁增厚 ,管腔变窄 ;咽鼓管粘膜上皮细胞胞浆淡染 ,粘膜下少量炎症细胞浸润 ,咽鼓管软骨镜下未见病理改变。结论 :豚鼠耳颞部受 6 0Gyγ射线照射后 ,中耳腔内发生化脓性炎症 ,随后局部出现肉芽组织及纤维组织 ,对中耳传音功能造成难以恢复的损伤 ;照射后鼓室粘膜比咽鼓管粘膜的损伤更严重 ;咽鼓管、鼓室粘膜下组织微循环减少 ,不利于上皮组织细胞的修复
Objective: To observe the microscopic morphological changes of tympanic mucosa of guinea pig’s eustachian tube after exposure to γ-rays, and to make a preliminary study on the pathological basis of its radiation injury. Methods: Sixteen normal adult guinea pigs were selected, and 60 gy γ-rays were received in the deafness. Four animals were sacrificed at 2, 4, 8 and 12 weeks after the end of irradiation. The middle ear cavity, eustachian tube, and tympanic mucosa of the irradiated side were observed with a microscope; 4 animals of the control group were not irradiated, and the tympanic mucosa and eustachian tube were taken. For normal control. Results: In the experimental group, a large number of pus cells appeared in the tympanic cavity, followed by localized granulation tissue and fibrous tissue; the tympanic mucosal epithelium was completely detached; the submucosal tissue was edema, the vessel wall was thickened, and the lumen became narrow; Eustachian tube mucosal epithelial cells The cytoplasm was lightly stained, a small amount of inflammatory cells infiltrated in the submucosa, and no pathological changes were observed under the eustachian tube cartilage. CONCLUSION: After the guinea pigs’ ear parts were exposed to 60 Gy gamma rays, purulent inflammation developed in the middle ear cavity. Granulation tissue and fibrous tissue then appeared in the guinea pigs’ ears, resulting in hard-to-recover damage to the middle ear. The tympanic mucous membrane was more affected than the eustachian drum after irradiation. The damage of the mucous membrane is more serious; the microcirculation of the submucosal tissue in the eustachian tube and tympanum is reduced, which is not conducive to the repair of epithelial cells.