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移植前组织培养能延长移植物在终宿主体内的存活时间,目前较普遍的是采用组织液培养,但这种培养方法有许多缺点:培养环境难以稳定,培养液易受污染,移植物易交性坏死,因此,有必要寻找一更可靠的培养方法。裸鼠是一种无胸腺鼠,没有T淋巴细胞,对异种移植物不排斥,近年广泛地被用于肿瘤学及免疫学研究,但应用于器官移植尚不多见。 Sollinger等人发现成人甲状旁腺(PTG)在裸鼠体内培养120天,PTG结构完好,但作为较理想的供体胎儿PTG能否在裸鼠体内存活尚无人知。本实验将胎儿PTG 23块分别在裸鼠肾包膜下培养10—30天,通过光镜及电镜观察,发现PTG结构完整,无变性坏死,无淋巴细胞浸润。应用裸鼠体内培养14天的胎儿PTG行临床移植治疗甲状旁腺功能低下症三例,移植疗效良好,证明经裸鼠体内培养14天的胎儿PTG仍可发挥较好的功能。另外,发现一例甲状旁腺瘤在裸鼠体内培养42天,其结构完整,无淋巴细胞浸润,与培养前的新鲜组织无明显差别。我们认为裸鼠可作为PTG移植物的一种理想培养工具,为移植体内培养开辟了一个可靠的新途径。
Tissue culture before transplantation can prolong the survival of the graft in the final host body, the current more common is the use of tissue culture, but this culture has many shortcomings: the culture environment is difficult to stabilize, the culture fluid susceptible to contamination, the graft is easy to sexual Necrosis, therefore, it is necessary to find a more reliable method of training. Nude mice, athymic mice, have no T lymphocytes and are not exclusive to xenografts. In recent years, nude mice have been widely used in oncology and immunology studies. However, application to organ transplantation is rare. Sollinger et al. Found that adult PTG was cultured in nude mice for 120 days and the structure of PTG was intact. However, whether or not PTG can survive in nude mice as an ideal donor remains unknown. In this experiment, fetal PTG 23 blocks were cultured in the nude mouse kidney capsule for 10-30 days, respectively. Through light and electron microscopy, we found PTG structure is complete, no degeneration and necrosis, no lymphocyte infiltration. Three cases of hypoparathyroidism were treated by transplantation of fetal PTG in nude mice for 14 days in vivo. The results showed that the transplanted PTG could still function well in nude mice cultured for 14 days. In addition, a case of parathyroid adenoma was found in nude mice cultured for 42 days, its structure is complete, no lymphocytic infiltration, no significant difference with the fresh tissue before culture. We believe that nude mice can be used as an ideal culture tool for PTG grafts, opening up a reliable new avenue for transplantation in vivo.