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以分子印迹作为识别体,制成高灵敏度和高选择性的化学发光传感器在线检测牛肉与鸡肉组织中残留的磺胺嘧啶。磺胺嘧啶作为靶分子,通过悬浮聚合制备了1～10μm的分子印迹聚合物。将分子印迹聚合物装入聚四氟乙烯管中,作为固相萃取柱,并联在八通阀上,用于在线选择吸附磺胺嘧啶。在最佳条件下,测得磺胺嘧啶线性范围7.0×10–9～9.0×10–7g/mL,方法的检出限为(3σ)2×10–10g/mL,11次平行测定3.0×10–8g/mL的磺胺嘧啶溶液的化学发光强度相对标准偏差为3.4%。方法能够用于检测肉类产品中残留的磺胺嘧啶。
Using molecular imprinting as an identifier, a chemiluminescent sensor with high sensitivity and selectivity was made to detect sulfadiazine residues in beef and chicken tissues on line. Sulfadiazine as a target molecule, prepared by suspension polymerization 1 ~ 10μm molecularly imprinted polymer. The molecularly imprinted polymer was packed in Teflon tube as solid-phase extraction column in parallel with the eight-way valve for on-line selective adsorption of sulfadiazine. Under the optimal conditions, the linear range of sulfadiazine was found to be 7.0 × 10-9 ~ 9.0 × 10-7g / mL. The detection limit of the method was (3σ) 2 × 10-10g / mL, and 11 times parallel determination of 3.0 × 10 The relative standard deviation of the chemiluminescence intensity of the solution of sulfadiazine at -8 g / mL was 3.4%. The method can be used to detect sulfadiazine residues in meat products.