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目的:研究雌激素及其拮抗剂对人胃腺癌SGC-7901细胞系血管内皮细胞生长因子(VEGF)表达的影响。方法:用雌二醇及其拮抗剂三苯氧胺(tamoxifen)处理体外培养的胃癌细胞系SGC-7901,在相差显微镜下观察细胞形态的变化;用四甲基偶氮唑蓝(MTT)比色法测定细胞增殖;用双抗体夹心酶联免疫吸附试验(ELISA)和免疫细胞化学SABC法测定细胞培养上清液和细胞质中VEGF蛋白含量。结果:胃癌细胞经雌二醇处理,培养液中VEGF浓度明显高于对照组(P<0.05);而雌二醇加三苯氧胺联合处理胃癌细胞,VEGF浓度有回降,比雌二醇处理组下降。结论:提示雌激素参与调节胃癌细胞VEGF的表达浓度,三苯氧胺有抑制VEGF表达的作用。
Objective: To study the effect of estrogen and its antagonist on the expression of vascular endothelial growth factor (VEGF) in human gastric adenocarcinoma cell line SGC-7901. Methods: The gastric cancer cell line SGC-7901 cultured in vitro was treated with estradiol and its antagonist tamoxifen, and the morphological changes of the cells were observed under a phase contrast microscope. MTT colorimetry Cell proliferation was detected by ELISA. Immunocytochemical SABC method was used to determine the content of VEGF in cell culture supernatant and cytoplasm. Results: The gastric cancer cells were treated with estradiol, the VEGF concentration in the culture medium was significantly higher than that in the control group (P <0.05); while the combination of estradiol and tamoxifen treatment of gastric cancer cells, the VEGF concentration decreased, compared with the estradiol treatment group . Conclusion: It is suggested that estrogen may regulate the expression of VEGF in gastric cancer cells and tamoxifen may inhibit the expression of VEGF.