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目的:通过丙酸氟替卡松(FP)对体外原代培养的鼻黏膜上皮细胞和体内鼻息肉组织中水通道蛋白5(AQP5)表达的影响,初步探讨AQP5在鼻息肉发病中的可能机制。方法:将人鼻黏膜上皮细胞进行体外原代细胞培养14天,传代后FP组细胞加入丙酸氟替卡松浓度为0.2%的完全培养液,非FP组细胞加入不含丙酸氟替卡松的培养液继续培养,7天后爬片固定,采用免疫组化技术检测细胞中AQP5的表达。并测定阳性细胞的光密度值和灰度值。将12例慢性鼻窦炎鼻息肉患者(均为初发型),3个月内无局部及全身激素使用史,取其鼻息肉组织,术前应用FP喷雾剂喷鼻7天,治疗7天后术中再次取其鼻息肉组织,术后病理均证实为鼻息肉组织,采用免疫组化技术检测鼻息肉组织中AQP5的表达和分布。并测定阳性细胞的光密度值和灰度值。结果:在体外培养的上皮细胞FP组的AQP5表达的灰度值明显降低(P<0.05),光度值则升高(P<0.05),在体内鼻息肉组织中,患者使用FP后鼻息肉体积明显缩小,,用药后AQP5在腺体细胞和淋巴细胞中表达的灰度值明显升高(P<0.05),光密度值则明显低于使用前(P<0.05)。结论:在FP作用下培养的鼻黏膜上皮细胞中的AQP5表达增加,可能解释在体情况下有利于组织间液的排除,减轻中鼻甲和下鼻甲的水肿,而在鼻息肉组织中,应用FP后,鼻息肉体积缩小,水肿减轻,可能与AQP5在腺体和淋巴细胞中表达减少有关,说明AQP5在鼻息肉的形成和发展过程中起到一定的作用。
OBJECTIVE: To investigate the effect of fluticasone propionate (FP) on the expression of aquaporin 5 (AQP5) in primary cultured nasal epithelial cells and nasal polyps in vitro and to explore the possible mechanism of AQP5 in the pathogenesis of nasal polyps. Methods: Human nasal mucosa epithelial cells were cultured in vitro for 14 days. After passage, FP cells were treated with fluticasone propionate 0.2% in complete medium. Non-FP cells were incubated with fluticasone propionate-free medium After 7 days, the slide was fixed and immunohistochemistry was used to detect the expression of AQP5 in the cells. The optical density and gray value of positive cells were determined. 12 patients with chronic sinusitis and nasal polyps (all initial type), no local and systemic hormones within 3 months of history, the nasal polyp tissue, preoperative application of FP spray nasal spray for 7 days, after 7 days of treatment The nasal polyps were taken again and the histopathology was confirmed as nasal polyps. The expression and distribution of AQP5 in nasal polyps were detected by immunohistochemistry. The optical density and gray value of positive cells were determined. Results: The gray value of AQP5 in FP group was significantly decreased (P <0.05) and the photometric value was increased in FP group (P <0.05). In the nasal polyps, the volume of nasal polyps (P <0.05). The optical density value of AQP5 in glandular cells and lymphocytes was significantly lower than that before use (P <0.05). CONCLUSION: The increased expression of AQP5 in nasal mucosa epithelial cells cultured under the action of FP may explain the exclusion of interstitial fluid in vivo and alleviate the edema of middle turbinate and inferior turbinate, while in nasal polyps tissue, FP After the nasal polyps reduce volume, edema alleviated, probably AQP5 expression in glands and lymphocytes decreased, indicating that AQP5 in nasal polyps formation and development play a role.