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目的研究远志的染色体核型及染色体特征。方法远志根尖用低温法处理,卡诺氏液固定,酸解去壁,改良品红染色、压片,显微摄像系统观察、拍照分析。结果远志的核型公式为K(2n)=2x=38=30m+8sm,属小型染色体,核型分类为2A型,不对称系数58.2%。结论为远志的分类、研究利用提供了依据。
Objective To study the chromosome karyotypes and chromosome features of Polygala tenuifolia. Methods Polygala root tips were treated with hypothermia, Carnot’s solution was fixed, acid solution to the wall, improved magenta staining, tabletting, microscopic imaging system observation, photograph analysis. Results Karyotype of Polygalaceae K (2n) = 2x = 38 = 30m + 8sm, is a small chromosome, karyotype is classified as type 2A, the asymmetry coefficient of 58.2%. Conclusion provides the basis for classification, research and utilization of Polygala.