采用高效液相色谱法测定重组人干扰素α1b粉雾剂的含量及均匀度。结果表明溶液中重组人干扰素α1b浓度在92.50～462.50μg/ml时峰面积(Y)与相应浓度(X)呈线性关系,(r=0.9999)。并且在成品的含量测定时,重组人干扰素α1b的色谱峰峰形良好,杂质与流动相无干扰。本方法精密度、准确度高,稳定性良好,能够准确、迅速的测定重组人干扰素α1b粉雾剂成品中的主药含量及均匀度。 Determination of content and uniformity of recombinant human interferon α1b powder by high performance liquid chromatography. The results showed that the peak area (Y) was linear with the corresponding concentration (X) (r = 0.9999) when the concentration of recombinant human interferon alb was 92.50 ~ 462.50μg / ml. And in the determination of the finished product, the recombinant human interferon α1b chromatographic peak shape good, impurities and mobile phase without interference. The method has the advantages of high precision, high accuracy and good stability, and can accurately and rapidly measure the main drug content and evenness in the finished product of the recombinant human interferon α1b powder aerosol.