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目的:建立肾康丸中大黄酚、大黄素的含量测定方法。方法:采用HPLC法,色谱柱为Diamonsi C18(250 mm×4.6 mm,5μm),流动相为乙腈-甲醇-0.1%磷酸溶液(42∶23∶35),流速为1.0 ml.min-1,检测波长为254 nm,进样量10μl。结果:大黄酚在9.38～150.00μg.ml-1(r=1.000 0),大黄素在3.31～53.00μg.ml-1(r=1.000 0)之间线性关系良好,其平均加样回收率大黄酚为99.83%,RSD为0.75%;大黄素为99.73%,RSD为0.34%。结论:此法简便,结果准确、灵敏,可用于肾康丸的含量测定。
Objective: To establish a method for the determination of chrysophanol and emodin in Shenkang pill. Methods: The HPLC method was Diamonsi C18 (250 mm × 4.6 mm, 5 μm). The mobile phase was acetonitrile-methanol-0.1% phosphoric acid solution (42:23:35) and the flow rate was 1.0 ml.min-1. Wavelength of 254 nm, injection volume 10μl. Results: There was a good linear relationship between the concentration of chrysophanol in 9.38-150.00 μg.ml-1 (r = 1.000 0) and emodin in the range of 3.31-53.00 μg.ml-1 (r = 1.000 0) Phenol 99.83%, RSD 0.75%; Emodin 99.73%, RSD 0.34%. Conclusion: This method is simple, accurate and sensitive, and can be used for the determination of Shenkang Pill.