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目的 研究血清丙型肝炎病毒(HCV) 含量与HCV 致病的关系及HCV 含量与抗HCV和丙氨酸转氨酶(ALT) 的相关性。方法 以逆转录聚合酶链反应(RTPCR) 法对HCV 感染的受血及相关供血系列血清进行HCVRNA定量分析,同时检测ALT 与抗HCV。结果 致输血后HCV 感染的供血中,HCVRNA 平均含量为108 .6 拷贝/L;抗HCV 及ALT 的异常检出率随HCVRNA 滴度升高而增加。结论 低含量HCV 供血即可导致输血后感染,病毒含量与抗HCV( +) 及ALT 水平有显著正相关,采用ALT 加抗HCV 联合筛检供血可明显降低输血后HCV 感染
Objective To study the relationship between serum hepatitis C virus (HCV) content and pathogenesis of HCV and the correlation between HCV content and antiHCV and alanine aminotransferase (ALT). Methods Reverse transcription polymerase chain reaction (RTPCR) was used to detect HCV RNA in sera of HCV-infected donors and related blood donors. ALT and antiHCV were also detected. Results After transfusion, the average HCV RNA in the blood supply of HCV infection was 108.6 copies / L, and the abnormal detection rate of anti-HCV and ALT increased with the increase of HCV RNA titer. Conclusions Low blood supply of HCV can lead to post-transfusion infection. The virus content is significantly and positively correlated with anti-HCV (+) and ALT levels. Combined use of ALT plus anti-HCV combined screening can significantly reduce post-transfusion HCV infection