目的　研究血清丙型肝炎病毒(HCV) 含量与HCV 致病的关系及HCV 含量与抗HCV和丙氨酸转氨酶(ALT) 的相关性。方法　以逆转录聚合酶链反应(RTPCR) 法对HCV 感染的受血及相关供血系列血清进行HCVRNA定量分析,同时检测ALT 与抗HCV。结果　致输血后HCV 感染的供血中,HCVRNA 平均含量为108 .6 拷贝/L;抗HCV 及ALT 的异常检出率随HCVRNA 滴度升高而增加。结论　低含量HCV 供血即可导致输血后感染,病毒含量与抗HCV( +) 及ALT 水平有显著正相关,采用ALT 加抗HCV 联合筛检供血可明显降低输血后HCV 感染 Objective To study the relationship between serum hepatitis C virus (HCV) content and pathogenesis of HCV and the correlation between HCV content and antiHCV and alanine aminotransferase (ALT). Methods Reverse transcription polymerase chain reaction (RTPCR) was used to detect HCV RNA in sera of HCV-infected donors and related blood donors. ALT and antiHCV were also detected. Results After transfusion, the average HCV RNA in the blood supply of HCV infection was 108.6 copies / L, and the abnormal detection rate of anti-HCV and ALT increased with the increase of HCV RNA titer. Conclusions Low blood supply of HCV can lead to post-transfusion infection. The virus content is significantly and positively correlated with anti-HCV (+) and ALT levels. Combined use of ALT plus anti-HCV combined screening can significantly reduce post-transfusion HCV infection