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目的:建立一种肺癌组织中微量元素亚细胞分布的分析方法。方法:采用电感耦合等离子体质谱法(ICP-MS)研究了肺癌组织、癌旁组织和正常肺组织中Cr、Mn、Fe、Co、Cu、Zn、Se、Cd 8个微量元素在细胞核、线粒体、溶酶体、微粒体和细胞上清液等亚细胞组分中的分布。结果:除Se元素外,其余各元素在亚细胞组分中均呈非均匀分布,采用t检验进行统计比较,所有待测元素在肺癌组织、癌旁组织和正常肺组织的亚细胞分布中均存在不同程度的显著性差异,在细胞核、线粒体、溶酶体、微粒体、细胞上清液等5个亚细胞组分中,肺癌组织中Cr、Mn、Fe、Co、Zn、Cd 6个元素的含量均低于癌旁组织和正常肺组织,而Se和Cu的含量高于癌旁组织和正常肺组织。结论:采用ICP-MS法可实现肺癌组织中微量元素亚细胞分布的准确分析,微量元素的变化与肺癌的发生、发展密切相关,对肺癌过程变化的研究和指导临床治疗均具有重要意义。
Objective: To establish a method for the analysis of the distribution of trace elements in lung cancer. Methods: Eight trace elements Cr, Mn, Fe, Co, Cu, Zn, Se and Cd in lung cancer tissues, adjacent tissues and normal lung tissues were studied by inductively coupled plasma mass spectrometry (ICP-MS) , Lysosomes, microsomes, and cell supernatants. Results: Except for Se element, all the other elements were non-uniformly distributed in subcellular fractions. Statistical analysis was performed by t-test. The subcellular distributions of all the elements tested in lung cancer tissues, paracancerous tissues and normal lung tissues There were significant differences in different degrees. Among the 5 subcellular components including nucleus, mitochondria, lysosome, microsome and supernatant, 6 elements of Cr, Mn, Fe, Co, Zn and Cd in lung cancer tissues The contents of Se and Cu were higher than those in paracancerous tissues and normal lung tissues. CONCLUSION: The accurate analysis of the distribution of trace elements and subunits in lung cancer tissues by ICP-MS method is very important. The changes of trace elements are closely related to the occurrence and development of lung cancer. It is of great significance to study the process changes of lung cancer and to guide the clinical treatment.