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目的:比较延胡索生品、用水炮制品、用醋炮制品中延胡索乙素在大鼠血浆及脏器中分布的差别,阐明药物在体内动态变化规律,为醋制增效理论的科学性提供实验依据。方法:血浆和组织匀浆样品采用液-液萃取法提取,用YMCC18柱(4.6mm×250mm,5μm)HPLC柱温30℃,流动相为甲醇-0.1%磷酸(三乙胺调pH6.0)(63∶37),流速0.8mL·min-1,检测波长230nm。结果:在大鼠血浆中醋制品中延胡索乙素的浓度在不同时间点均高于生品和水制品,在肝脏中2,3,4h延胡索乙素的浓度均高于生品和水制品。结论:大鼠灌胃延胡索不同炮制品水煎液后,醋制品中延胡索乙素在血浆及肝脏中浓度最高,这一结果为揭示“延胡索醋制增效”传统理论的科学内涵积累实验数据。
OBJECTIVE: To compare the distribution of tetrahydropalmatine in rat plasma and organs between crude drug products from Yanhusheng, products from processed water cannons, and processed vinegar, and to clarify the dynamic changes of drugs in vivo, and to provide experiments for the scientificity of vinegar boosting theory. in accordance with. Methods: The plasma and tissue homogenate samples were extracted by liquid-liquid extraction. The column temperature was 30 °C on a YMCC18 column (4.6 mm x 250 mm, 5 μm). The mobile phase was methanol-0.1% phosphoric acid (triethylamine adjusted to pH 6.0). (63:37), flow rate 0.8 mL·min-1, detection wavelength 230 nm. RESULTS: The concentration of tetrahydropalmatine in rat vinegar products was higher than that of raw products and aquatic products at different time points. The concentrations of tetrahydropalmatine at 2, 3, and 4 h in the liver were higher than those of raw and aquatic products. Conclusion: After intragastric administration of decoction of different processed products in rats, the concentration of tetrahydropalmatine in vinegar products is highest in plasma and liver. This result is to reveal the scientific connotation accumulation experiment of traditional method of “enhanced efficacy of vine vinegar”. data.