论文部分内容阅读
目的建立冠脉宁片中血竭素的含量测定方法。方法采用反相高效液相法(RP-HPLC),分析柱:Agilent Eclipse XDB-C18(150mm×46mm,5μm),以乙腈-0.05mol·L-1磷酸二氢钠(55∶45)为流动相,流速控制在1.0 ml·min-1,检测波长为440nm。结果在9~144μg·ml-1范围内,血竭素与峰面积有良好的线性关系(r=0.999 7)。加样回收率为99.3%,RSD=1.57%。结论所建立的方法简便,准确,快速,可用于冠脉宁片中血竭药材的质量控制。
Objective To establish a method for determination of dracorhodin in Guanmaining tablets. Methods RP-HPLC was used. The analytical column was Agilent Eclipse XDB-C18 (150mm × 46mm, 5μm) with acetonitrile-0.05mol·L-1 sodium phosphate monobasic (55:45) Phase, flow rate control at 1.0 ml · min-1, detection wavelength of 440nm. Results In the range of 9 ~ 144μg · ml-1, there was a good linear relationship between dracorhodin and peak area (r = 0.999 7). The sample recovery was 99.3%, RSD = 1.57%. Conclusion The established method is simple, accurate and rapid, and can be used for quality control of blood-dried herbs in Guanning Capsules.