【摘 要】
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Aim To determine the role of receptor component protein (RCP) in calcitonin gene-related peptide (CGRP) and angiotensin Ⅱ signal transduction in action of vascular peroxidase-1 (VPO1) in VSMC.Methods
【机 构】
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Institute of Pharmacy and Pharmacology, University of South China , Hengyang , Hunan 421001, China
【出 处】
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第十二次全国动脉硬化性疾病学术会议
论文部分内容阅读
Aim To determine the role of receptor component protein (RCP) in calcitonin gene-related peptide (CGRP) and angiotensin Ⅱ signal transduction in action of vascular peroxidase-1 (VPO1) in VSMC.Methods Mouse vascular smooth muscle cell line A10 (A10VSMC) was employed in the study.Western blot was used to detect the expression of RCP and VPO1 protein.RT-PCR was used to detect the expression of RCP and VPO1 mRNA.RCP-specific small interference RNA (RCP-siRNA) was used to silence oligonucleotide sequence co-immunoprecipitation was used to determine interaction of RCP and Gαs/Gβ/Gγ Results Both CGRP and angiotensin Ⅱ up-regulated the expression of RCP while CGRP down-regulated the expression of RCP induced by angiotensin Ⅱ in VSMC;Angiotensin Ⅱ up-regulated the expression of VPO1 in a concentration and time-dependent manner in VSMC;CGRP inhibited the expression of VPO1 induced by angiotensin Ⅱ and the effect of CGRP was enhanced by Catalase, H2O2 scavenger.RCP-siRNA successfully decreased RCP protein expression by ~80% in 48 h, following with an decrease in VPO1 expression in CGRP and/or angiotensin Ⅱ induced VSMC In addition, RCP co-immunoprecipitated with Gαs and Gβ but not with Gγ in VSMC.Conclusion RCP may contribute to the inhibitory effect of CGRP on angiotensin Ⅱ induced VPO1 expression in VSMC, and the mechanism may be related to the interactions between RCP and Gαs / Gβ.
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