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Objective To investigate the changes of mutation of HPRT exposure to γray and benzopyrene,in order to try to find new differential diagnosis methods for cell injury induced byγray and benzo-pyrene.Methods Human peripheral blood samples from three personswere exposed to 60Co γ-rays at 10 different doses (0-5Gy) and benzo-pyrene at 6 differentconcentrations (0-10μg/ml), its DNA was isolated from white cell.