【摘 要】
：
目的：研究常用调节免疫功能中药黄芪对60Coγ射线照射小鼠T淋巴细胞亚群、血常规检测以及免疫器官指标的影响,筛选抗辐射黄芪有效提取物.
方法：采用总剂量为4Gy的60Coγ射
【机 构】
：
山西大学,山西太原030006;山西省中医药研究院,山西太原030012山西省中医药研究院,山西太原030012
论文部分内容阅读
目的：研究常用调节免疫功能中药黄芪对60Coγ射线照射小鼠T淋巴细胞亚群、血常规检测以及免疫器官指标的影响,筛选抗辐射黄芪有效提取物.
方法：采用总剂量为4Gy的60Coγ射线一次性全身照射雌性昆明小鼠,造成60Coγ放射损伤小鼠模型.小鼠在照射前7d及照射后7d,分别以黄芪水煎液和黄芪30％、60％、90％乙醇提取物,连续灌胃14d,每日1次,检测给药后小鼠外周血液学指标变化,采用流式细胞术分析T淋巴细胞亚群CD4+、CD8+的变化.
结果：给药各组外周血PLT和WBC均高于辐射模型对照组(P＜0.05、0.01);各给药组与模型对照组比较,黄芪水煎液组CD4+增加明显(P＜0.01),其他各组无显著性差异(P＞0.05),各给药组CD8+无明显变化(P＞0.05),CD4+/CD8+有增加趋势,黄芪水煎液组有显著性差异(P＜0.05).
结论：黄芪不同提取物对辐射致60Co小鼠T淋巴细胞亚群功能损伤有不同程度的保护作用,其中黄芪水煎液效果最佳.
其他文献
Somatic embryogenesis receptor kinase(SERK)proteins play pivotal roles in regulation of plant development and immunity.Rice genome contains two SERK genes OsSer
Salt stress exerts negative effects on plant growth,development and yields,with roots being the primary site of both perception and damage.Salix matsudana(Chine
Abscisic acid(ABA)is a key endogenous phytohormone in plant development and responses to various stresses.In this study,a transcription factor OsRE1 was identif
Transcription factors such as many members in basic leucine Zipper(bZIP)family have been shown to play important roles in regulating drought responses in plants
Rice is sensitive to chilling stress,especially at the seedling stage.In the process of molecular breeding,functional markers are very useful for enhancing the
Lesion mimic mutant lmm4 was identified from an EMS-induced Nipponbare mutant library.Brown lesions were first observed on the tip of lmm4 leaves at initial til
A unique cDNA fragment was isolated by suppressive subtractive hybridization(SSH)from a tea plant subjected to herbivory by the tea geometrid Ectropis obliqua,c
本研究以C57BL/6J ES细胞系为背景,旨在筛选制作模式动物的最佳供体鼠.本实验采用C57BL/6J系ES细胞,以C57BL/6J-Tyrc-2J白化小鼠的囊胚为受体,注射同一种阳性ES细胞,比较其成
目的：建立有效的钩端螺旋体PCR检测方法,并对树鼩、长爪沙鼠和灰仓鼠等三种新型实验动物进行感染情况调查.
方法：针对NCBI公布的钩端螺旋体序列,设计并筛选特异性引物,优化
目的：了解各种水的水质状况对健康的影响.方法：采用组1太湖源水、组2太湖自来水、组3长江源水、组4长江自来水和组5阴性对照农夫山泉水、组6阳性对照农夫山泉水加有3、4-苯并芘