【摘 要】
：
Canonical transient receptor potential (TRPC) channels are Ca2+-permeable, non-selective cation channels that are widely expressed in mammalian cells.Various molecules have been found to regulate TRPC
【机 构】
：
Department of Physiology,Seoul National University College of Medicine,Seoul,South Korea
【出 处】
：
International Conference for Physiological Sciences 2012(201
论文部分内容阅读
Canonical transient receptor potential (TRPC) channels are Ca2+-permeable, non-selective cation channels that are widely expressed in mammalian cells.Various molecules have been found to regulate TRPC both in vitro and in vitro, but it is unclear how heterotrimeric G proteins transmit external stimuli to affect TRPC5 function.Here, we demonstrated that TRPC5 was regulated in a bidirectional manner by the Gαs regulatory pathway.We found that the whole-cell TRPC5 current was increased by treatment with the β-adrenergic receptorspecific agonist isoproterenol (246 ± 36%, n=6), the adenylatecyclase activator forskolin (273 ± 6%, n=5), and the membrane permeable cAMP analogue 8-Br-cAMP (251 ± 63%, n=7).In addition, we observed robust Ca2+transients induced by isoproterenol utilizing a Ca2+ imaging technique in HEK293 cells.After pretreatment with Gαs signaling cascade agonists or effectors, the membrane localization of TRPC5 was significantly increased by treatment with ISO (155 ± 17%, n=3), FSK (172 ± 39%, n=3) or 8-Br-cAMP (216 ± 59%, n=3).However, when GTPγS or GTPγS and IP3 were included in the pipette solution, the ISO-induced TRPC5 current was attenuated or inhibited, respectively.In conclusion, we suggest that the Gαs pathway regulates TRPC5 not only via the inhibition of PKA phosphorylation but also through potentiating actions that are dependent on the facilitation of intracellular Ca2+ dynamics and increased channel trafficking.
其他文献
Autophagy of vascular endothelial cells (VECs) protects against VEC injury, which is a critical step in atherosclerosis.Previously, we found that small molecule 6-amino-2, 3-dihydro-3-hydroxymethyl-1,
Nedd4 family interacting protein 1 (Ndfip1), an adaptor protein for Nedd4-mediated ubiquitination, has been found to play an essential role in regulating divalent metal transporter 1 (DMT1) and iron h
Manufactured nanoparticles are currently used for many fields.However, their potential toxicity provides a growing concern for human health.Previously, we prepared novel magnetic nanoparticles (MNPs),
Macrophage recruitment to sites of inflammation is an essential step in host defense.However, the signals regulating their mobilization are still not fully understood.Here, we investigated the role of
To explore the possible action of protein kinase C (PKC) in the developmental and maladaptive plasticity after spinal cord injury (SCI) of neonatal rats, 40 neonatal rats (7-14 days old) were used to
A small conductance Ca2+-activated K+ (SK) channels, SK2 channel, is important in configuration of action potential in atrial myocytes.Here, we determined the potential role of RyRs on SK2 channel.Met
Caveolin-3 (Cav 3) is a muscle specific scaffolding protein that is the main structural element of caveolae, cholesterol rich membrane microdomains that form mechanically deformable invaginations and
Background and Objective: Negative feedback is common in biological processes and can increase a systems stability to internal and external perturbations.The homeostasis is one of the five most import
We reported previously that Cl- channels play important roles in regulation of cell proliferation.Using the molecular and patch-clamp techniques, it was proved in this study that the Cl-channel protei
Catecholaminergic polymorphic ventricular tachycardia (CPVT) is an arrhythmogenic cardiomyopathy that manifests as recurrent syncope and sudden cardiac death triggered by high adrenergic tone.Mutation