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Objective To study the construction of tissue engineering adipose in vitro with silk fibroin scaffold and human umbilical cord mesenchymal stem cells (hUCMSCs)transfected by recombinant human insulin gene lentivirus.Methods 1.hUCMSCs infected with recombinant lentiviral pLenti6(.)3-insulin-IRES-EGFP(Group A) by the best MOI (MOI=10)were seeded in silk fibroin scaffold;hUCMSCs transfected by EGFP gene (Group B)was regarded as negative control;and then the cell-scaffold complexes were cultured in adipogenic differentiation medium.2.