【摘 要】
：
Objective Nerve regeneration is a complex biological phenomenon incorporating multiple cells, growth factors and an extracellular matrix.MiRNAs are endogenous, non-coding 21-to 23-nucleotide small RNA
【机 构】
：
Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, China
【出 处】
：
中国神经科学学会第九届全国学术会议暨第五届会员代表大会
论文部分内容阅读
Objective Nerve regeneration is a complex biological phenomenon incorporating multiple cells, growth factors and an extracellular matrix.MiRNAs are endogenous, non-coding 21-to 23-nucleotide small RNA molecules that regulate gene expression by binding to the 3 untranslated region of target mRNAs, leading to their translational inhibition or degradation.The present study is to explore whether miRNAs are capable of mobilizing the intrinsic capacity for neurite growth and promoting axon regeneration.Methods We analyzed the changes in miRNA and mRNA expression in the DRG, following sciatic nerve transection, using microarray and bioinformatics analysis.To validate the microarray platform, we assessed the expression by real time Taqman PCR.To validate the target, we performed luciferase reporter assay.Further, we transfected the miRNA-21 mimics and PITX2 siRNA to the cultured DRG neurons.Results (1) The bioinformatics analysis indicated that miRNA-21 play important roles in regulating nerve regeneration via the target PITX2 in the network.(2) miRNA-21 is inversely correlated with PITX2 expression in DRG tissues.(3) In the cultured DRG neurons, miRNA-21 promotes neurite growth and inhibits PITX2 expression.Transfection of PITX2 siRNA also promotes neurite growth.Conclusion miRNA-21 is significantly up-regulated following sciatic nerve injury and promotes neurite growth by targeting PITX2.
其他文献
Regulation of neuronal NMDA receptor is critical in synaptic transmission and plasticity.Protein kinase C (PKC) promotes NMDA receptor (NMDAR) trafficking to the cell surface via interaction with NMDA
PKCε has fundamental roles in the actions of neurite outgrowth and pathfinding abilities of growth cone.The activity radius of growth cone can be altered by PKCs, meanwhile, the morphology response of
Objective The bee venom test has long been used to evaluate the antinociceptive, antihyperalgesic and anti-inflammatory effects of endogenous and exogenous substances, however, the dose-effect of subc
Self-avoidance is a mechanism by which dendrites from the same neuron repel one another in order to establish uniform coverage of the dendritic field.The importance of self-avoidance for the developme
Objective Grb2-associated binder (Gab) docking proteins have crucialroles in neural development.However, the roles of Gab proteins in the development of synapses remain elusive.Here, we investigate th
Objective Precise regulation of cyclin-dependent kinase 5 (Cdk5), a member of the cyclin-dependent kinase family, is critical for the proper neuronal development and functions.Cdk5 is activated throug
为了研究在成年动物脑内缺血诱导的纹状体新生神经元是否能在结构上整合到现有的神经环路中，在动物缺血再灌注第1和11周将荧光金(一种逆向示踪剂)注射到黑质网状部，我们通过标记BrdU并结合免疫组化的方法标记新生神经元，同时应用激光共聚焦扫描的方法研究纹状体内新生神经元的投射情况。结果显示，正常或缺血再灌注的动物脑内均可在同侧检测到FG阳性信号。并且，在缺血再灌注第12周缺血侧纹状体的新生神经元上检测到
Targeted gene expression is a powerful approach in studies of the nervous system.We have used To12 mediated enhancer trap to find tissue specific enhancers in zebrafish genome and to produce transgeni
Objective Oligodendrocyte precursor cells (OPCs) differentiate into oligodendrocytes (OLs), which are responsible for myelination.However, the molecular mechanisms of maturation and myelination by oli
How much neocortical development depends on connections remains elusive.Here, we show that Celsr3|Dlx mutant mice have no extrinsic neoeortical connections yet survive to postnatal day 20, acquire a b