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Background: Introduce high throughput drug screening technology research progress.Methods: According to the papers published in recent years, this paper introduced relative knowledge about medicine researching and high throughput screening method.At last, application of this technology was stated.Results: Cell level screening is in a natural living cell conditions, close to the biochemical process of the body, and have much more targets, index to detect and can shorten the cycle of drug discovery.Conclusions: High throughput drug screening technology has broad prospects .