Objective: Poly(β-amino) esters (PBAEs) exhibit great potential as gene delivery reagents because they are easily synthesized and exhibit the relatively low toxicity and high transfection efficiencies in a wide variety of cell types.Pullulan (PL) is a water-soluble, non-toxic, non-immunogenic, non-mutagenic and non-carcinogenic nature polysaccharide which produced by Aureobasidium pullulans.Due to its excellent biological and physicochemical features, pullulan has become an attractive ingredient for many pharmaceutical applications and chemical manipulations.