Multiplex gene assay is a valuable molecular tool not only in academic science but also in clinical diagnostics.Multiplex PCR assays,DNA microarrays,and various nanotechnology-based methods are examples of major techniques developed for analyzing multiple genes; none of these,however,are suitable for point-of-care diagnostics,especially in resource-limited settings [1-2].This report shows that loop-mediated isothermal amplification (LAMP) of nucleic acid can be integrated on a multiplex microfluidic etched ITO electrode for real-time,quantitative electrochemical detection.We simultaneously monitored the amplification process of three bacteria DNA on the basis of RT-PCR by measuring and analyzing the electrochemical signal of methylene blue (MB) with eight channel etched ITO electrode.Etched ITO electrode was integrated with PDMS chip,thus the sealed environment is formed.The MB reduction current on the chip with the amplicon significantly reduced compared to non-amplified control [3].This assay with the ability of analyzing multiple genes qualitatively and quantitatively is highly specific,operationally simple,and cost/time effective with the detection limits less than 15 copies/μL within 45 minutes.We successfully developed an electrode chip for identifying three important bacteria that cause upper respiratory tract infection.