【摘 要】
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Smut is a fungal disease which is the widespread popularity in sugarcane planting areas.Early detection and accurate identification of Sporisorium scitamineum are essential in smut management practice
【机 构】
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Key Laboratory of Sugarcane Biology and Genetic Breeding,Fujian Agriculture and Forestry University,
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Smut is a fungal disease which is the widespread popularity in sugarcane planting areas.Early detection and accurate identification of Sporisorium scitamineum are essential in smut management practice.In this paper,four specific primers targeting the core effector of Pep1 gene in S.scitamineum revealed by its genome sequence were designed.Three impact factors of Mg2+,primer and Bst DNA polymerase,which are the components of the reaction system and may affect the products of LAMP,were studied by the methods of a single factor experiment and the L16(45) orthogonal design.
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