Aim: To determine the content of ehlorogenic acid in YIN TIAN granules by HPLC.Methods: the chromatographic conditions were as follows: Platisil-ODS (250 mm×4.6 mm, 5 μm) was used as the chromatographic column, and acetonitrile-0.2% phosphoric acid solution (20 ∶ 90) was used as the mobile phase.The flow rate was 1.0 mL·min-1 and the detection wavelength was 326 nm.Results:The concentration linear range of chlorogenic acid was 5.4~108 μg·mL-1 (r=0.9997).The average recovery rate was 99.05% (RSD=-2.65%, n=6).Conclusion: The method appeared to be simple, reliable, accurate and can be used to the quality control of YIN TIAN granules.