【摘 要】
：
AIM Coptisine is an isoquinoline alkaloid extracted from Coptidis Rhizoma.This study aims to elucidate if coptisine is responsible for cardioprotection using myocardial infarction (MI) and investigate
【机 构】
：
Beijing Key Laboratory of Drug Targets Identification and Drug Screening,Chinese Academy of Medical
【出 处】
：
第二届世界天然药物和传统药物药理学学术会议
论文部分内容阅读
AIM Coptisine is an isoquinoline alkaloid extracted from Coptidis Rhizoma.This study aims to elucidate if coptisine is responsible for cardioprotection using myocardial infarction (MI) and investigate its mechanism.METHODS Rats were randomized into 7 groups: normal, isoproterenol (Iso), Iso + fasudil, Iso + isosorbide dinitrate and Iso + coptisine (25, 50, 100 mg· kg-1).Rats pretreated with coptisine for 21 d (ig) and injected subcutaneously with high-dose Iso (85 rag· kg-1) on the 20th, 21st day at an interval of 24 h.Cardiac function and biomarkers of cardiac ischemia were assessed after MI.RESULTS Coptisine has strong antioxidant activity, and it can maintain cell membrane integrity, ameliorate mitochondrial respiratory dysfunction, reduce myocardial cells apoptosis, inhibit RhoA/ROCK expression in experimental MI rats.CONCLUSION The present study provides experimental evidence that coptisine has strong antioxidant activity, and it can maintain cell membrane integrity, ameliorate mitochondrial respiratory dysfunction and improve cardiac systolic/diastolic dysfunction induced by Iso.Inhibition of RhoA/ROCK pathway was involved in coptisine induced cardioprotection effects on experimental MI.Coptisine should be considered as a novel adjunctive therapy for attenuating myocardial damage.
其他文献
AIM The present study was designed to obverse whether c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) regulates the angiogenesis of human umbilical vein endothelial cells (HUVEC) in
AIM Silent information regulator 1 (Sirtl), a class ⅢI histone deacetylase, has been reported to attenuate myocardial and kidney ischemia-reperfusion(I-R) injury.However, the mechanisms of Sirtl in he
AIM Interactions of tumor cells with endothelial cells (EC), for example tumor cell adhesion and migration through the EC layer, are essential components of tumor metastasis.Intracellular calcium regu
AIM To create a novel mouse model of combined hyperlipidemia using a modeling agent (MA) made of a mixture of schisandrin B, cholesterol and bile salt (1,2 and 0.5 g·kg-1) suspended in olive oil 20 ml
AIM To investigate the effects of dietary intake of Fructus Schisandrae chinensis (FSC), both aqueous and ethanolic extracts (AqFSC, EtFSC), on serum/hepatic lipid contents in normal diet (ND)-and hig
The low success rate, despite a huge capital investment, has made the research and development of conventional drugs very costly.Over the past few decades, researchers have focused on drug discovery f
AIM To evaluate the impact of JTN on beta cell function in mice with impaired glucose tolerance (IGT).METHODS Two animal models with IGT were chosen to evaluate the effect of JTN on beta cell function
AIM This study was designed to assess the antinociceptive effect of verticinone and to evaluate the involvement of opioid receptors activation.METHODS In vivo nociceptive response was induced by hot-p
AIM To observe the inhibition of the extract of Radix scutellariae and Trollius chinensis on four kinds of influenza viruses in vitro.METHODS Cytopathic effect (CPE) observation was used to observe th
Anglotensin Ⅱ (Ang Ⅱ) plays a key role in the regulation of blood pressure and the process of cerebral ischemia.We have previously demonstrated that pinocembrin (5,7-dihydroxyflavanone), one of the ma