Diabetes is a metabolic disease characterized by high blood glucose and caused by absolute or relative lack of insulin.The treatment of diabetes is multi-component and multi-path,however,the therapeutic mechanism is unclear as yet.In this study,the potential bioactivities of about 20 kinds of traditional Chinese medicines in treating diabates were investigated in three aspects to be as follows:the activity in inhibiting advanced glycation end products(AGES)formation,the activity in inhibiting lipid peroxidation and the α-glucosidase inhibitory activity.The results showed that the Gardenlae Fructus had good anti-lipid peroxidation activity,and Scutellarlae Radix all had a certain activity in three vitro active experiments.Combine with related literatures,we chosed Scutellarlae Radix and Gardenlae Fructus made an intensive studies on the extraction and active ingredients.Geniposide,iridoids from Gardenlae Fructus,and the extractions of Scutellarlae Radix and Gardenlae Fructus,were investigated for faeces metabonomics of type 2 diabetic rats by using UPLC-QTOF/MS combined with PCA and OPLS-DA method.A type 2 diabetic model was obtained by the gastric gavage of fat emulsion and injecting STZ separately for the different group rats for 12 weeks,and the faeces were collected termly for testing.The total ion flow diagram showed that each drug had activity to regulate the metabolism of T2DM rats towards the normal rats,and the PCA results indicated that the disturbance appeared in each groups of rats in vivo metabolism.Region distance of model group and drug group in the OPLS scatter diagram showed that the drug stated above has a certain therapeutic effect on diabetic rats.According to the 36 potential biomarkers which we analyzed and identified,we could see the changes of amino acid metabolic pathways,fatty acid synthesis pathways,sphingolipid metabolism pathways,bile acids and other metabolites.Geniposide,iridoids from Gardenlae Fructus,the extractions of Scutellarlae Radix and Gardenlae Fructus could adjust amino acid metabolic pathways and bile acids with different degrees,but had little effects to fatty acid synthesis pathways and sphingolipid metabolism pathways.This research could provide the bases for the faeces metabonomics by using UPLC-QTOF/MS method.