Development of a trypsin-immobilized monolithic polymer with pipette-tip format for protein digestio

来源 :第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会 | 被引量 : 0次 | 上传用户:wosee_2008
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  In this work, trypsin immobilized onto a macroporous polymer monolith in a pipette tip for digesting proteins has been developed to explore the utility of using a tip-based protein digestion methodology in a bioanalytical setting.Prior to the in situ polymerization, the surface of the polypropylene (PP) tips were modified to allow covalent attachment of the monolithic polymer to the pipette tip.1 Following this, the porous poly(butyl methacrylate-ethylene glycol dimethacrylate) (BMA-EDMA) monolith was fabricated in situ in polypropylene tips by UV initiation.2,3 Pipette tips with the monolith inside were photografted with 1-vinyl-4,4,dimethylazlactone (VAL) to provide the pore surface with reactive functionalities required for trypsin immobilization.Following reaction with trypsin an enzymatic microreactor was created with high proteolytic activity.
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