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that pGlycoNovo is suitable for identification of N-glycopeptides from different species.We also showed that pGlycoNovo could be used to identify the modifications on the glycans in fission yeast samples.pGlycoNovo showed a good performance for the identification of O-glycopeptide compositions.By using the de novo algorithm,glycan composition of the glycan part of an O-glycopeptide can be identified quite well,but the glycosylation sites are not that easy to be determined in HCD-MS/MS spectra.